Trypsin 0.5ukatal/mg

1.EP standard 2.Porcine or Bovine pancrease 3.Food&pharmaceutical grade trypsin 4.Assay:Not less than 0.5 ukatal/mg

Trypsin  0.5 ukatal/mg

CAS 9002-07-7                                 

Source Porcine (Bovine) pancreas

General description

Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23300 dalton, and is a single peptide chain composed of 223 amino acid residues. With rigorous specificity, the trypsin specifically acts on the peptide linkage constituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation also reduces or loses gradually. It is easily soluble in the water, but insoluble in trichloromethane, ethanol, ether and glycerin, and the optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated when boiled for a short time; if the salt is added into the hot solution, the protein will precipitate, and the enzyme action of filtrate cannot be seen, and Ca2+ plays the role in protecting and activating the trypsin.

The high purity Trypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by ultra-filtration.

Items	Specification	Method
Description	white crystalline lyophilized powder	EP6.0
Identification	A) A purple colour develops B) No colour develops with in 3 min of mixing	EP6.0
Appearance of solution	Solution S is not more opalescent than reference suspension 3	EP6.0
Absorbance	1) 280nm : 13.5 ~ 16.5 2) 250nm : not more than 7.0	EP6.0
PH	3.0-6.0	EP6.0
Histamine	Not more than 1µg/0.2µkatal	EP6.0
Loss on drying	Not more than 5%	EP6.0
Limit of trypsin	Not more than 1%	EP6.0
Microbial limits Total viable aerobic count Salmonella  E Coli	≤10000cfu/g Negative Negative	EP6.0
Assay	Not less than 0.5 ukatal/mg, calculated on the dried basis	EP6.0

Storage

 Sealed, Dark, at temperature 2-8°C

Trypsin 0.5ukatal/mg