- Chemical Reagents[10]
- Organic Intermediate[10]
- Pharmaceutical[2]
- Animal Extract[2]
- Contact Person : Ms. Li Christina
- Company Name : Beijing Geyuantianrun Bio-Tech Co., Ltd.
- Tel : 86-10-60279131
- Fax : 86-10-60279131 60270523 Address: Beijing Daxing DistrictBiomedical Industry Base Tianfu Road NO.3 Zip: 102609 Country/Region: China (Mainland) Province/State: Beijing City:
- Address : Beijing,Beijing,Beijing Daxing DistrictBiomedical Industry Base Tianfu Road NO.3
- Country/Region : China
- Zip : 102609
Chymotrypsin 1500u/mg
Chymotrypsin
CAS 9004-07-3
Source Bovine (Swine) pancreas
General description
Chymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).
Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.
The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Chromatography and ultra-filtration.
Specification
Items | Specification | Method |
Appearance | White crystallized powder | USP33 |
Identification | No purple color develops within 3 minutes | USP33 |
Loss on drying | No more than 5.0% | USP33 |
Residue on Ignition | No more than 2.5% | USP33 |
Microbial limits |
| |
Pseudomonas aeruginosa | Absent | USP33 |
Salmonella | Absent | USP33 |
Staphylococcus aureus | Absent | USP33 |
Limit trypsin | Not more than 1%. | USP33 |
Assay | NLT 1000 USP Chymotrypsin Units/mg (calculated on the dried basis) and 90.0%-110.0% of the labeled potency. | USP33 |
Storage
Sealed, Dark, at temperature 2-8°C
Items | Specification | Method |
Description | white crystallize powder | EP6.0 |
Identification | A) A purple colour develops B)No colour develops with in 3 min of mixing | EP6.0 |
Appearance of solution | Solution S is not more opalescent than reference suspension 2 | EP6.0 |
Absorbance | 1) 281nm : 18.5 ~ 22.5 2) 250nm : not more than 8.0 | EP6.0 |
PH | 3.0-5.0 | EP6.0 |
Histamine | Not more than 1µg/5.0µkatal | EP6.0 |
Loss on drying | Not more than 5% | EP6.0 |
Limit of trypsin | Not more than 1% | EP6.0 |
Assay | Not less than 5.0 ukatal/mg, calculated on the dried basis | EP6.0 |
Storage
Sealed, Dark, at temperature 2-8°C
Chymotrypsin 1500u/mg
